Using the polymerase chain reaction and standard recombinant DNA techniques, a series of new multipurpose low-copy-number (lcn) vectors, pWSK29, pWKS30, pWKS129 and pWKS130, have been constructed. Plasmids pWSK29 and pWKS30 carry the ampicillin-resistance marker (ApR), 20 unique cloning sites flanke …
Molecular cloning is a set of experimental methods that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with...
SLIC cloning enables precise and sequence independent engineering and is based on joining vector and insert with 15-25 bp homologies on both DNA ends by homologous recombination.
In Sillico TA cloning with SimVector. SimVector makes it so easy to design TA cloning experiments. The TA cloning wizard accepts both commercial T-vectors or a restriction designed T-vector to combine them with the modified PCR product and generates a highly accurate recombinant DNA plasmid map.
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Cloning vectors provide a backbone for the DNA insert to be reproduced and propagated in bacteria; however, these vectors are only useful for storing a genetic sequence. By themselves, they are incapable of allowing for transcription and translation of the gene into a functional protein product.
Simply select your three PCR products generated earlier with attB sites, the corresponding 3 pDONR vectors and the pDestHaw destination vector and go Cloning → Gateway Cloning. The Gateway cloning tool will confirm the selected sequences can be “reacted” and create the same 15,894 bp pDESTHaw-based expression plasmid.
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Aug 11, 1997 · The cloning capacity in this system is up to 8 kb so far. Because of the high efficiency of the cosmid cloning system and lack of wild-type adenovirus contamination, the cosmid adenoviral vector cloning method is simple and efficient to develop recombinant adenoviral vectors for human gene therapy.