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BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, etc. BioVisions products are currently ...

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High- and low-copy-number shuttle cloning vectors were constructed by incorporating the Escherichia coli P15A plasmid origin of replication into the pAMβ1-derived vectors, pIL252 and pIL253. The resulting vectors were structurally stable in Lactococcus, which is a common feature of theta-replicating plasmids, and also displayed good structural ...

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Problem to use 2 μmplasmid as a cloning vector. • First, there is the question of a selectable marker. Some yeast cloning vectors carry genes conferring resistance to inhibitors, such as methotrexate and copper, but most of the popular yeast vectors make use of a radically different type of selection system.

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Vector Selection Tool We've made finding the right vector for your research easier. Simply search by keyword or filter your results by vector type, host system, or cloning system. Once the initial results are displayed, they can be further refined by selecting among characteristics such as promoter, inducer, and antibiotic selection.

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This is the biotechnology questions and answers section on "Cloning Vectors" with explanation for various interview, competitive examination and entrance test. Solved examples with detailed answer description, explanation are given and it would be easy to understand.

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We would like to inform you about our new vectors for Sequence and Ligation Independent Cloning (SLIC), an advanced version of the Ligation Independent Cloning method (LIC). Introduction. LIC is a cloning method that makes use of annealing of single-stranded complementary overhangs on the target vector and a PCR-generated insert of at least 12 ...

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Vectors for Cloning Download PDF Info Publication number US20090042249A1. US20090042249A1 US12/167,527 US16752708A US2009042249A1 US 20090042249 A1 US20090042249 A1 ...

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Cloning Vectors and Kits. Includes an array of cloning and subcloning vectors, and the convenient Flexi® Cloning Systems for protein expression. Molecular Biology Enzymes and Reagents. Find ligases, polymerases, PNK, phosphatases, nucleases and more. Molecular Weight Markers

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Cloning vector - a DNA molecule that carries foreign DNA into a host cell, replicates inside a bacterial (or yeast) cell and produces many copies of itself and the foreign DNA.

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The best selection of Royalty Free Cloning Vector Art, Graphics and Stock Illustrations. Download 12,000+ Royalty Free Cloning Vector Images.

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This session will cover 1) what restriction enzymes are and how they cut DNA, 2) the different types of restriction enzymes and the advantages and disadvantages of using them, and 3) how restriction enzymes are used to create a recombinant DNA molecule.
Jan 21, 2009 · Cloning Vectors for Rice Cloning Vectors for Rice Kim, Sung-Ryul; Lee, Dong-Yeon; Yang, Jung-Il; Moon, Sunok; An, Gynheung 2009-01-21 00:00:00 J. Plant Biol. (2009) 52:73–78 DOI 10.1007/s12374-008-9008-4 ORIGINAL RESEARCH Sung-Ryul Kim & Dong-Yeon Lee & Jung-Il Yang & Sunok Moon & Gynheung An Received: 5 December 2008 /Revised: 9 December 2008 /Accepted: 9 December 2008 /Published online: 21 ...
All vectors were derived from pCambia T-DNA cloning vectors, with the exception of a chemically inducible vector, for Agrobacterium sp.-mediated transformation of a wide range of plant species.
Apr 17, 2015 · Cloning Tips for Restriction Enzyme-Digested Vectors and Inserts Posted on April 17, 2015 April 17, 2015 by Sara Klink Cartoon created and owned by Ed Himelblau While T-vector cloning is commonly used for PCR-amplified inserts, restriction enzymes still have their uses.
Vectors used for cloning contain a large number of restriction enzyme sites clustered in one region. Which term is used to describe this region on the vector? palindrome (or palindromic region) consensus sequence (or consensus region) polycloning site? selectable marker site (or selectable marker region) transformation region

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Here, we provide a set of 34 ligation- independent cloning (LIC) binary vectors for expression analysis, protein localization studies, and misexpression that will be made freely available. This set of plant LIC vectors offers a fast alternative to standard cloning strategies involving ligase or recombination enzyme technology.
Oct 12, 2009 · donor vectors (kan/chlor resistant; grow in ccdB-tolerant cells) 218 pDONR221 middle donor vector; attP1-P2 flanking chlor/ccdB cassette: Invitrogen: 1.1: 219 pDONRP4-P1R 5' donor vector; attP4-P1R flanking chlor/ccdB cassette: Invitrogen: 1.1: 220 pDONRP2R-P3 3' donor vector; attP2R-P3 flanking chlor/ccdB cassette: Invitrogen: 1.1: transposase clones 396